Concept prototype. Website redesign with an AI Method Finder (demo mode). Not the official HELIX site.

Mixed-mode HPLC columns & method development

Separate what
single-mode columns can’t.

Amaze, Coresep and Heritage columns combine reversed-phase, HILIC and ion-exchange in one stationary phase — so isomers, ions and polar compounds resolve without ion-pairing reagents.

Browse columns
860+
compounds in the HELIX chromatogram library
3
column families: Amaze, Coresep, Heritage
4
retention mechanisms: RP, HILIC, cation- and anion-exchange
METHOD FINDER AI · DEMO

Answers only from published HELIX application notes · shows what is verified, hypothesised and missing

01 · Columns

Three families. One idea:
more than one interaction.

HelixFlash Flash columns: normal-phase, RP, ion-exchange, mixed-mode Kits Mixed-mode method development kits: HILIC, RP, RP + HILIC Standards LC & GC standards, reference materials, reagents

02 · AI Method Finder

An application scientist that never
makes up a retention time.

Describe your analytes, sample and detector. The Method Finder searches HELIX’s published chromatograms, extracts exact conditions, and separates every answer into three levels of evidence.

  1. VERIFIED

    Published by HELIX

    Column, mobile phase, flow, detector and chromatogram — copied from the application note, with the source linked.

  2. HYPOTHESIS

    Scientifically reasoned

    e.g. transferring an ELSD method to LC-MS: volatile buffer ✓, flow too high for ESI ✗. Marked as a starting point, never as a validated method.

  3. MISSING

    What we still need

    Sample matrix, instrument, quantitation goal. The assistant asks — and hands a complete brief to a HELIX scientist.

Try a real question →

03 · Applications

Published chromatograms,
now searchable by meaning.

04 · Services

When there’s no published method,
we develop one.

A

HPLC method development

An experienced HELIX scientist is assigned to your project. NDA available on request. “Avoid the mess.”

B

Mixed-mode training

Seminars and trainings on mixed-mode chromatography for your team, from fundamentals to method transfer.

Seminars & trainings
C

Publishing service

HELIX pays for published articles and research that involve HELIX products.

Learn more
D

Column & silica recycling

Return used columns and silica gel through the HELIX recycling program.

Program details

05 · Technology

Mixed-mode vs.
ion-pairing.

Ion-pairing reagents contaminate systems, suppress MS signal and are slow to equilibrate. Mixed-mode phases put the ionic interaction on the surface instead — and let you tune retention with three levers.

  • ACN %Hydrophobic / HILIC retention
  • Buffer mMIon-exchange strength
  • pHIonization of analyte & phase

Tell us what you need to separate.

Concept brief

What an AI Method Finder does for HELIX

24/7 first-line application support

Answers “which column, which conditions?” instantly, in any language, from your own published notes — no invented numbers.

Qualified leads, not contact forms

Every conversation ends with a structured brief: analytes, matrix, detector, goal, recommended column. Sales sees it in the Team view.

Demand map for new applications

Questions with no published method show exactly which application notes and columns customers are looking for.

Method development upsell

When nothing is published, the assistant explains the gap and routes the customer into paid method development.

Demo mode: answers are generated in the browser from a curated index of real HELIX application notes. Production version: full library + LLM with citations, CRM integration, analytics.